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Image Search Results
Journal: Cellular and Molecular Immunology
Article Title: Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2 341–456 fusion protein
doi: 10.1038/cmi.2011.21
Figure Lengend Snippet: In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) and T2 cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Article Snippet: The
Techniques: In Vitro, Lactate Dehydrogenase Assay, Purification, Control
Journal: Cellular and Molecular Immunology
Article Title: Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2 341–456 fusion protein
doi: 10.1038/cmi.2011.21
Figure Lengend Snippet: Induction of Her2-specific Th1 responses and CTLs in HLA-A2.1/Kb transgenic mice immunized with Hsp70L1–Her2341–456-pulsed DCs. Splenocytes from immunized HLA-A2.1/Kb transgenic mice were used to assess Her2-specific immune responses by a CTL assay. The splenocytes were stimulated with Her2341–456 and then used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) cells and Her2-E75 peptide pulsed T2 cells were used as Her2-specific and HLA-A2.1-restricted targets (T), with SK-BR-3 and T2 cells as control targets. Various E/T ratios were tested as indicated. Data are presented as mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Article Snippet: The
Techniques: Transgenic Assay, CTL Assay, Control