tap deficient hybridoma cell line t2 Search Results


97
ATCC t2 cell line
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
T2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
ATCC human tap deficient t2 cell line
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Human Tap Deficient T2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
EuroClone t2 tap-deficient hla-a2-positive cell line
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
T2 Tap Deficient Hla A2 Positive Cell Line, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t2 tap-deficient hla-a2-positive cell line - by Bioz Stars, 2026-08
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CEM Corporation tap-deficient t2 cells
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Tap Deficient T2 Cells, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CEM Corporation cell line t2
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Cell Line T2, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza amaxa cell line nucleofector kit c
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Amaxa Cell Line Nucleofector Kit C, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
ATCC hla a2 tap deficient cell t2
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Hla A2 Tap Deficient Cell T2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CEM Corporation t2 cells 174 cem.t2 hybridoma
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
T2 Cells 174 Cem.T2 Hybridoma, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
ATCC antigen processing tap deficient human t2 cell line39
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Antigen Processing Tap Deficient Human T2 Cell Line39, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tap+deficient+hybridoma+cell+line+t2/pm18337841-101-20-30?v=ATCC
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90
CEM Corporation mutant tap-deficient cell line t2
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Mutant Tap Deficient Cell Line T2, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CEM Corporation tap-deficient t2 (cem × 721.174.t2) cell line
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
Tap Deficient T2 (Cem × 721.174.T2) Cell Line, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC t2 cells
In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells <t>(Her2+,</t> <t>HLA-A2.1+)</t> and <t>T2</t> cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.
T2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) and T2 cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.

Journal: Cellular and Molecular Immunology

Article Title: Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2 341–456 fusion protein

doi: 10.1038/cmi.2011.21

Figure Lengend Snippet: In vitro induction of human Her2-specific CTL responses by Hsp70L1–Her2341–456-pulsed DCs. PBLs from healthy donors were stimulated with autologous DCs pre-pulsed with Hsp70L1–Her2341–456 or Her2341–456 to assess the Her2-specific CTL responses by a standard 4-h lactate dehydrogenase (LDH) release assay. CD8+ CTLs purified from the PBLs stimulated with three rounds of DCs pulsed with Hsp70L1–Her2341–456 or Her2341–456 were used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) and T2 cells pulsed with Her2-E75 were used as target cells (T). Control targets included SK-BR-3 cells (Her2+, HLA-A2.1−), irrelevant CAP-1 peptide pulsed or unpulsed T2 cells. Various E/T ratios were tested as indicated. Data are presented as the mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.

Article Snippet: The T2 cell line (TAP-deficient, HLA-A2.1 + ), human colorectal adenocarcinoma cell line SW620 (Her2 + , HLA-A2.1 + ) and human breast adenocarcinoma cell line SK-BR-3 (Her2 + , HLA-A2.1 − ) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured according to its instructions.

Techniques: In Vitro, Lactate Dehydrogenase Assay, Purification, Control

Induction of Her2-specific Th1 responses and CTLs in HLA-A2.1/Kb transgenic mice immunized with Hsp70L1–Her2341–456-pulsed DCs. Splenocytes from immunized HLA-A2.1/Kb transgenic mice were used to assess Her2-specific immune responses by a CTL assay. The splenocytes were stimulated with Her2341–456 and then used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) cells and Her2-E75 peptide pulsed T2 cells were used as Her2-specific and HLA-A2.1-restricted targets (T), with SK-BR-3 and T2 cells as control targets. Various E/T ratios were tested as indicated. Data are presented as mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.

Journal: Cellular and Molecular Immunology

Article Title: Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2 341–456 fusion protein

doi: 10.1038/cmi.2011.21

Figure Lengend Snippet: Induction of Her2-specific Th1 responses and CTLs in HLA-A2.1/Kb transgenic mice immunized with Hsp70L1–Her2341–456-pulsed DCs. Splenocytes from immunized HLA-A2.1/Kb transgenic mice were used to assess Her2-specific immune responses by a CTL assay. The splenocytes were stimulated with Her2341–456 and then used as effector cells (E), and SW620 cells (Her2+, HLA-A2.1+) cells and Her2-E75 peptide pulsed T2 cells were used as Her2-specific and HLA-A2.1-restricted targets (T), with SK-BR-3 and T2 cells as control targets. Various E/T ratios were tested as indicated. Data are presented as mean±s.e.m. of three independent experiments. CTL, cytotoxic T lymphocyte; DC, dendritic cell; HLA, human leukocyte antigen; HSP, heat shock protein.

Article Snippet: The T2 cell line (TAP-deficient, HLA-A2.1 + ), human colorectal adenocarcinoma cell line SW620 (Her2 + , HLA-A2.1 + ) and human breast adenocarcinoma cell line SK-BR-3 (Her2 + , HLA-A2.1 − ) were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured according to its instructions.

Techniques: Transgenic Assay, CTL Assay, Control